Journal: Journal of Biomedical Science

Article Title: EpCAM aptamer mediated cancer cell specific delivery of EpCAM siRNA using polymeric nanocomplex

doi: 10.1186/s12929-014-0108-9

Figure Lengend Snippet: Schematic showing the cell specific silencing strategy mediated by PEI nanocomplex fabricated with aptamer and siRNA. PEI nanoparticle is formed using sodium citrate as charge stabilizer, followed by the addition of siRNA and EpCAM aptamer to form the PEI-Apt-siRNA complex. This complex guided by the aptamer, binds to the EpCAM positive cells and delivers the siRNA in the cytoplasm resulting in target gene silencing and inhibition of cellular function pertained to it.

Article Snippet: PEI nanocomplex has been efficiently used for the delivery of siRNA using target specific antibody or affibody or aptamer.

Techniques: Inhibition, Cell Function Assay

Journal: Journal of Biomedical Science

Article Title: EpCAM aptamer mediated cancer cell specific delivery of EpCAM siRNA using polymeric nanocomplex

doi: 10.1186/s12929-014-0108-9

Figure Lengend Snippet: Effect of citrate on the nanocomplex size, charge and characterization of the nanocomplex. Graph showing the hydrodynamic sizes (A) and surface charge (B) of PEI: citrate nanocomplexes formed using different ratio of PEI to citrate measured using zetasizer. C . Titration of different concentration of aptamer and siRNA was carried out and loaded onto 2% agarose gel with ethidium bromide and checked for the retention of the PEI complex on the wells. Lane 3 shows 200nM of aptamer and 200nM of siRNA is required to saturate 0.3μgs of PEI and the next highest concentration of 300nM of aptamer & siRNA respectively had some amount of free siRNA and aptamer (lane 4). Lane 5 & 6 indicates free aptamer and siRNA indicated with red and black arrow respectively. On the right, histogram plot showing Particle size distribution of the PEI-Apt-siRNA nanocomplex. D . Histogram overlay plot showing the percent number distribution of the PEI nanocore alone and PEI-nanocomplex with aptamer and siRNA (hydrodynamic diameter in nm) measured using zetasizer. E . Graph showing the total counts of representative zeta-potential (mV) of the PEI nanocore and the PEI-Apt-siEp nanocomplex. F . TEM images of the PEI nanocomplex left panel showing the uniformity of particle distribution and histogram showing distinct particles with a spherical shape (G) . H . Graph showing the percentage cell proliferation upon treating with different concentration from 0.1 to 3 μg/mL of PEI on MCF-7 and WERI-Rb1 cell line till 48 h. Inhibitory effect of PEI on the cell proliferation and mitrochondrial activity was assessed by MTT assay.

Article Snippet: PEI nanocomplex has been efficiently used for the delivery of siRNA using target specific antibody or affibody or aptamer.

Techniques: Titration, Concentration Assay, Agarose Gel Electrophoresis, Zeta Potential Analyzer, Activity Assay, MTT Assay

Journal: Journal of Biomedical Science

Article Title: EpCAM aptamer mediated cancer cell specific delivery of EpCAM siRNA using polymeric nanocomplex

doi: 10.1186/s12929-014-0108-9

Figure Lengend Snippet: Cell Uptake of the PEI nanocomplex by MCF-7 and WERI-Rb1 cells. The fabricated PEI complexes and free aptamer were added to MCF-7 cells (upper panel A - E ), WERI-Rb1 cells (lower panel A - E ) and incubated for their uptake at 37°C for 4 h followed DAPI counterstaining and microscopic evaluation. Images were taken at 40× using AxioObserver fluorescent microscope. Legend on the top of phase image represents the aptamer or nanocomplex added to the respective panels.

Article Snippet: PEI nanocomplex has been efficiently used for the delivery of siRNA using target specific antibody or affibody or aptamer.

Techniques: Incubation, Microscopy